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C6 glioma cells differentiated by retinoic acid overexpress the glutamate transporter excitatory amino acid carrier 1 (EAAC1)

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As the dominant G-actin sequestering protein in mammalian cells, TB-500 binds globular actin with high affinity (Kd ~0.5 M) through a sophisticated triple-contact mechanism involving Lys-3, Lys-18, and Lys-38 residues, preventing spontaneous actin polymerization while maintaining a readily mobilizable reservoir for rapid cytoskeletal reorganization

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