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The standard protocol begins at 0.25 mg per week for the first four weeks, then escalates to 0.5 mg, then 1.0 mg, with some protocols going up to 2.4 mg per week for weight management

Anesthesiology 101:13631371 Adachi T, Weisbrod RM, Pimentel DR, Ying J, Sharov VS, Schneich C, Cohen RA (2004) S-glutathiolation by peroxynitrite activates SERCA during arterial relaxation by nitric oxide

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Therefore, not only mammalian cell lines but also model organisms such as Saccharomyces cerevisiae , Drosophila melanogaster , Caenorhabditis elegans , and Arabidopsis are used to understand human diseases (Xu and Mller 2011