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The experiment was divided into a normal control group, a DSS model group, and a DSS+IGF-1 LR3 treatment group (with doses of 0.1mg/kg, 0.5mg/kg, and 1mg/kg, respectively, orally administered daily), and treated continuously for 7 days
1c) serves as a control to ensure that equal amount of D2R was present in each sample and that the differences in co-immunoprecipitation of DAT were due to changes in the degree of D2R-DAT interaction, rather than antibody artifacts
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