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The copper ion is chelated primarily through the imidazole nitrogen of histidine and the alpha-amino nitrogen of glycine, creating a stable, biologically active complex

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Therefore, the findings are not completely consistent across species, likely because humans are long-lived while mice have much shorter lifespans, resulting in different disease time courses and inflammatory milieu

The enzyme glutathione reductase (GR) , utilizing the coenzyme NADPH, steps in to break the disulfide bond, recycling GSSG back into the active, reduced GSH state