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cerevisiae in which the ATP consuming glucose-glycogen bypass pathway had been shut-down by deletion of the GBE achieved 3.1-fold higher ATP-generating activity and 1.7-fold higher GSH productivity compared with the control strain [15]

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8 weeks: ~2 vials 12 weeks: ~23 vials 16 weeks: ~3 vials Insulin Syringes (U-100, 1 mL) Per injection: 1 syringe 8 weeks (once daily): ~56 syringes 16 weeks (once daily): ~112 syringes Bacteriostatic Water (10 mL bottles) Use ~3.0 mL per 10 mg vial for reconstitution

Cells were passaged upon reaching 80% confluency using Trypsin-EDTA (Lonza, Switzerland)