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One-way ANOVA and Tukeys multiple comparison tests were performed using the IBM SPSS Statistics 26 software

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2021;14(1):e003128

Equal enzyme input amounts were applied into plate wells coated with (i) either fully assembled biotinylated TMV Cys /Bio adapter sticks, or (ii) released lower-order CP Cys /Bio aggregates, (iii) or stoichiometric amounts of maleimide-PEG 11 -biotin linkers, respectively, or into (iv) uncoated plates resulting in mere enzyme adsorption (Figure 6A)