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Acute exposure to methamphetamine alters TLR9-mediated cytokine expression in human macrophage

The therapeutic potential of this axis is underscored by our finding that dual inhibition of endogenous serine synthesis (via PHGDH inhibitors) and exogenous serine uptake synergistically overcomes Np63-mediated resistance, providing a novel strategy for this therapeutically challenging subtype

Cells were then stained with secondary antibodies (goat anti-mouse IgG H&L (Alexa Fluor 647), Abcam, ab150115-1001 and goat anti-rabbit IgG H&L (Alexa Fluor 488), Abcam, ab150077-1001) at 1:1,000 in BK buffer at room temperature for 30 min, followed by staining with DAPI at 1:10,000 in PBS

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