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After blocking with blocking solution (0.2% casein, 0.3% Triton X-100, and 3% goat serum diluted in PBS) for 2 h at room temperature, anti-C3 (1:100 dilution) antibody-stained sections were reacted overnight at 4 C

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2.2 Participants 2.2.1 Inclusion, eligibility, and exclusion criteria All participants were healthy Japanese residents aged between 45 and 65 years

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