Cell proliferation (doubling time) and viability were measured by trypan blue dye exclusion and cell counting, as well as with the CellTiter 96 Aqueous One Assay (Promega), according to the manufacturers instructions
The other significant difference is that the blue pens in the UK have four color options such as blue, red, purple, and gold
However, last November, LeRoy Collins, the former Governor of Florida and the current president of the National Association of Broadcasters, who has the reputation of being a maverick in the broadcasting business, made a speech before a group of broadcasters in Portland, Oregon, in which he suggested that, because of what he called mounting evidence that tobacco provides a serious hazard to health, broadcasters had a moral responsibility to consider taking corrective action against the televising of some types of cigarette commercials, notably those featuring well-known athletes and those expressly designed to influence young people
However, electronic cigarettes are slightly less perceived as dangerous by the general public, whether they are traditional devices (78%) or puffs (85%)
