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The efficiency of an Agrobacterium -mediated gene transformation protocol can be determined through the percentage of regenerated plants in the selective medium, the molecular methods employed (percentage of PCR-verified plants and percentage of plants verified through advanced molecular techniques such as RT-PCR and Southern blot), and the expression methods used

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A new tobacco levy should be directed into a smoke-free fund, a resource used primarily to achieve a smoke-free UK by funding the measures and services that will help people to stop smoking and prevent them from starting