For targets with no existing fluorophore conjugated antibody the following primary antibodies were used: anti NRF2 polyclonal (1:200, Thermofisher, PA5-27882)
Anyone considering peptides should consult a healthcare professional, particularly individuals with underlying health conditions, those on medication, or women who are pregnant or breastfeeding.
Briefly, cells were sequentially treated with the medium containing 25 M of CldU (Sigma-Aldrich, C6891) and then 250 M of IdU (Sigma-Aldrich, I7125) for 20 min
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