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Proline concentration was determined on the basis of a standard curve (050 g mL), and the value was calculated using the formula: mole proline/g FW = [(g proline/mLmL toluene) / 115.5 g/mole] / [(g sample) /5] Hydrogen peroxide content in soybean leaves was determined according to Velikova et al

The percentage that reaches systemic circulation intact is estimated to be quite low, though exact figures for GHK-Cu specifically aren't well established in published literature