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PNGase F assay To remove N-linked oligosaccharides on PLA2G2F, 20 g of lysate and the corresponding volume of concentrated conditioned medium were adjusted subsequently for the following buffer: 0.25% SDS, 50 mM dithiothreitol, preheated at 95 C for 5 min, 50 mM Tris-HCl (pH 7.5) and 1% NP-40

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Cells were collected, and OMs were disrupted with glass beads and examined by TEM for the presence of nozzles 54