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Materials and methods Cell culture RAW264.7 cells were obtained from the Second Hospital of Shandong University and cultured in DMEM medium (Genview) supplemented with 10% fetal calf serum (Applied Biosystems) in a humidified, 5% CO 2 atmosphere at 37 C

Reynolds or at whatever outside studio produced the Vantage campaign graphics were working from the same formal vocabulary, with access to the same print technology, and with a brief that demanded the same result: an image that creates optical depth, visual movement, and immediate arrest of the viewer's attention

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The menthol flavour may make smoking feel smoother and less harsh to the throat, but it does not reduce health risks