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These invisible micro-thaw cycles gradually promote: Ice crystal reformation Aggregation Hydrolysis Structural stress Analytical Findings Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) showed a significant reduction in intact peptide purity compared with freshly prepared aliquots

It can attack polyunsaturated fatty acids (PUFAs) and produce lipid peroxides (lipid-OOHs) and their reactive degradation products [72, 75]

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doi: 10.1073/pnas.84.9.3033 5 ValletPGGunternRHofPRGolazJDelacourteARobakisNKet al