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In addition, it increases the number of tumor-infiltrating CD8 + T cells to inhibit tumor immune escape (Fig

The resulting PCR products were purified using AxyPrep Mag PCR Clean-Up Kit (Axygen, United States), validated by an Agilent 2,100 Bioanalyzer (Agilent Technologies, United States), and quantified using a Qubit 2.0 Fluorometer (Invitrogen, United States)