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doi: 10.1289/EHP1673 118

Every time he appears, he sarcastically tells the main character that it's all pointless and then asks 'Are you alone now?', and he is really alone.[121] When he answers that he is alone even though there are other people, he gives a characteristic sarcastic response and breaks down the door and kills the main character, leading to the ending of Yes, I am alone .[122][123] It seems to be a murder of humans who are wandering around the main character's house, and it seems to be a powerful visitors that are not comparable to any other visitors, such as the teenagers who are nicely wandering, as well as a large number of armed soldiers

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Then, each slide was treated with 50 L of CMA3 staining solution and incubated in darkness for 30 min at room temperature (RT)