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The solution was transferred at room temperature into a 3 L round bottom flask, diluted with 260 mL of methanol and 760 mL of water, treated rapidly with 15 mL of a freshly prepared aqueous solution of sodium borohydride (0.145 g, 3.83 mmol) under vigorous stirring that causes the formation of a dark brown colloidal suspension

10.1021/bi100698n 112 PaulsenC

Evidence suggests that RGD enhances cell attachment and spreading of osteoblasts onto scaffolds and graft material [140, 141, 159, 188] whilst increasing cellular proliferation and the expression of ALP, Runx2, osteocalcin, osteopontin and bone sialoprotein [141, 142, 146, 190, 191]