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This was the very question Tuell and colleagues set out to explore

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This controlled inflammation leads to: Increased fibroblast activity Enhanced collagen deposition Strengthening of the weakened ligaments Improved joint stability and function Unlike steroid injections, which suppress inflammation and may degrade tissue over time, prolotherapy seeks to stimulate tissue regeneration

It is also used to decrease the concentrations of inflammatory cytokines (IL-6, IL-18) as well as involved in increasing the level of serum Ca 2+ ions