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Iron overload, through ROS production, promotes cellular senescence in surrounding cells, thereby reinforcing a self-sustaining cycle of fibrosis, inflammation, and senescence [27]

These changes were not due to the limitations of the Langendorff mouse heart model as we found comparable results on analysing in vivo ischaemia in the heart using the left anterior artery, descending (LAD) occlusion model (Supplementary Fig