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Symptoms may develop gradually and include fatigue, weakness, constipation, loss of appetite, weight loss, numbness or tingling in the hands and feet, difficulty maintaining balance, depression, confusion, and poor memory

However, the most common are ROS (superoxide, hydroxile, alcoxile, peroxile, hydrogen peroxide) and reactive nitrogen species (RNS) (nitric oxide, nitrogen dioxide, peroxinitrile)

After reconstitution, the solution should be used immediately or divided into aliquots and frozen

Given many of these uses require serious or emergency situations, calcium gluconate should only be used under the direction and supervision of a licensed veterinarian