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Blood samples retrieved as part of the routine screening for latent Mycobacterium tuberculosis at Statens Serum Institut (SSI, Copenhagen, Denmark) and the Department of Respiratory Diseases B, and the Department Clinical Microbiology, Aarhus University Hospital (Aarhus, Denmark) were collected from 01 September 2009 to 30 March 2011 (9217 patients)

Amyotrophic lateral sclerosis (ALS) 9

Bound FcRn was detected either by a horseradish peroxidase (HRP)-conjugated anti-GST antibody from goat (Rockland Immunochemicals, 200-301-200) (diluted 1:5000 in PBS/T/S) or streptavidin-AP (diluted 1:5000 in PBS/T/S) and visualized by addition of TMB substrate (CalBiochem) or p-nitrophenylphosphate (AP substrate) (Sigma-Aldrich) diluted to 10 g/mL in diethanolamine buffer

Loss of Efficacy: This is the most insidious sign because it's not visually apparent