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50 l supernatant was then transferred to black clear bottom optical 96-well Viewplates (Perkin Elmer) and fluorescent intensity was determined at 485 nm excitation/510 nm emission using an Envision plate reader (Perkin Elmer)

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Post-vaccination changes in gene expression are normally associated with resolution of the acute inflammatory process, which involves activation of NFE2L2, the gene encoding the antioxidant transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2)