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ECG Recording In deeply anesthetized rats, an ECG was recorded continuously in all three main leads by positioning stainless steel electrodes on all four limbs using an ECG monitor by 2090 Medtronic programmer (Minneapolis, MN, USA) connected to a digital oscilloscope LeCroywaverunnerLT342 (Chestnut Ridge, NY, USA), which enabled precise recordings, measurements and analysis of ECG parameters