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The specimens were processed on paraffin wax, and 5-m-thick paraffin sections were obtained and stained with hematoxylin and eosin or immunohistochemically for FVIII (Dako, Glostrup, Denmark) using Dako Autostainer, according to the manufacturers protocols

When reconstituted with bacteriostatic water and stored correctly in a refrigerator (2-8C), IGF-1 LR3 should remain stable and potent for at least 30 days, and potentially up to 45 days

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Os benefcios mais bem documentados nos estudos pr-clnicos incluem: acelerao da cicatrizao de tendes e ligamentos (com melhora das propriedades biomecnicas), proteo da mucosa gstrica contra AINEs e lcool (reduo de 78% da superfcie ulcerosa em modelos animais), estimulao da angiognese via VEGF, e efeitos neuroprotetores em modelos de leso cerebral