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Description

In human SH-SY5Y neuroblastoma cells, a well-established model for studying neuronal behavior, Epitalon significantly increased the secretion of soluble amyloid precursor protein (sAPP) by ~20%

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The coding region was cloned into a modified pETM-11 bacterial expression vector which was derived from a pET-24d(+) vector (Novagen) by insertion of a tobacco etch virus (TEV) protease cleavage site following an N terminal hexa-histidine and protein A tag