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Dehydroepiandrosterones antiepileptic action in FeCl 3 -induced epileptogenesis involves upregulation of glutamate transporters

For 3-MST , CSE , UCP3 and beta-actin, RT-PCR amplification reaction was performed in a volume of 20 l containing 0.9 l Gene Expression Assay (Thermo Fisher Scientific), 10 l TaqMan Fast Universal PCR Master Mix and 2 l cDNA

This is the reason that GSH varies less in the menstrual cycle than the steady states of GSH would suggest GSH: glutathione Because GSH changes very slowly when estradiol and S change, the GSH concentration at each time is a weighted average of both low and high GSH concentrations corresponding to many different S values

The homogenates were centrifuged at 600 g for 10 min at 4 C