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Carbamidomethylation was set as a fixed modification, whereas methionine oxidation, asparagine and glutamine deamination, and glycosylation options were set as variable modifications, according to published data (Lebede et al., 2021)

121 Mis-metalation of Fur by Mn II under conditions of Mn II toxicity, in contrast, is detrimental to cell viability, and is avoided by proper tuning of the coordinated responses of the Mn II -sensing repressor and Fur to distinct ranges of cellular metal activity (Figure 8A)