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Quantification of mitochondrial superoxide was similarly executed except that the samples were stained with 5 M MitoSox TM Red (Thermo Fisher Scientific)

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2BPC157 The BPC157 peptide synthesis process of the pilot scale of embodiment 2 12020g(Loadding1.0mmol/g)1TBTU/HOBt-24gHPLC751C 18 (Kromasil-C 18 30mm*250mm)13.6gHPLC98 Referring to the specific process of Example 1, the difference is that all reagents are directly amplified 20 times, that is, 20 g of Wang's resin (Loadding: 1.0 mmol/g) is weighed for reaction, and the TBTU/HOBt reaction of Example 1 is also used to prepare the resin - Peptide conjugates, cleaved to prepare crude peptides, a total of 24 g of crude products were obtained, with a purity of 75% as determined by HPLC