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Considering extensive previous studies reporting that PVN neuronal activity is closely associated with gastrointestinal function 27,28 , we performed immunofluorescence staining to identify the neuronal types in the PVN marked by EGFP: ~ 92% of EGFP + cells were co-stained with a glutamate-specific antibody, while only 12% of EGFP + cells were co-localized with a GABA-specific antibody (Fig

Further insights into determining optimal intervention timing derive from the study by Parkin et al

Understanding these differences helps clarify what to expect from each approach and why treatment timelines vary

Couple that with some MLD as talked about above and theyll be down in no time