Briefly, sodium bicarbonate, glucose, sodium pyruvate, and all 20 amino acids (except cystine and serine), biotin (B4639, Sigma), and vitamin B12 (V6629, Sigma) were added to the DMEM medium (#D9800-28, USBiological, Salem, MA) to match standard IMDM, supplement with 15% human AB serum and 15% dialyzed FBS (#SH300790340, Cytiva Hyclone), along with 10 ng/mL interleukin-3, 50 ng/mL stem cell factor and 2IU/mL erythropoietin
Non-small cell lung cancer (NSCLC) accounts for 85% to 90% of all lung cancers, 386 and is a common type of lung cancer, with lung adenocarcinoma being the most prevalent subtype within NSCLC
The experimental protocol was approved by the Institutional Animal Care and Use Committee of Nanjing First Hospital, Nanjing Medical University (license number: SYXK (Su) 2021-0007)
It is also worth noting that the impact of CBS on tumor growth and progression is context-dependent, with CBS functioning as an oncogene in colon, ovarian and breast cancer but as a tumor suppressor in liver cancer 62,64
