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The authors noted that the damaging effects of homocysteine begin to appear between 8 and 10 mol/L.36 Hyperhomocysteinemia may increase the risk of Alzheimers disease by compromising the integrity of the bloodbrain barrier, allowing toxic substances to pass through to brain tissue

Next, the eluted proteins were concentrated using protein concentrator vivaspin 6 (10,000 MWCO) columns

Samples for measurements with GSH were also supplemented with 1 U GR and 100 M NADPH to remove traces of GSSG and to maintain a highly reducing redox state

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