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Incubations with primary antibody (anti-Cystatin C, Sigma, HPA013143, used at 1:100 dilution) were performed at room temperature for 30 min

The Finnish HELMI cohort had previously been analyzed using 16S rRNA gene amplicon sequencing 77 and for five of the SFB-positive samples the metagenomic data was available (Supplementary Data 4, Fig

L.SulkavaR.PekkanenJ.ErkinjunttiT.EhnholmC.KivinenP.et al (1998)

9 Furthermore, the accumulation of free radicals damages the biological components of proteins, lipids, and DNA, as it breaks down single and double strands of DNA, degrades nitrogenous bases, and transforms and cross-links DNA with proteins