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Some patients come weekly, others monthly, or as needed for travel, stress, or immune support

Traditional protein detection methods, such as enzyme-linked immunosorbent assay (ELISA) (Hayrapetyan et al., 2023), and immunohistochemistry (IHC) (Ramos-Vara, 2005), have significant limitations when processing complex proteomes

The disulfide bond between cysteine molecules is vital for the stability of structural proteins like keratin, found in hair, skin, and nails

1 Introduction Inflammatory bowel disease (IBD) in humans comprises at least two chronic inflammatory intestinal disorders, defined as Crohns disease (CD) and ulcerative colitis (UC)