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Insulin resistance and heart failure: molecular mechanisms

Genomic HA-tagged TBC1D9B HeLa Cells were generated with CRISPR/Cas9 mediated knockins using Alt-R sgRNA and ssODN Donors from IDT (Coralville, IA, USA) with the following sequences: Alt-R sgRNA: AGGCATCAGCCGGAAACTCC ssODN Donor: TGATGACACCTTGGGCCAGGCCTCACAGCTGCAGGCATCAGGCGTAGTCGGGGACGTCGTAGGGGTAGCCGGAAACTCCAGGCTGCTCATGGTCACTGGCGGTGCTGAACTGTCTC