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After 30 min incubation at room temperature, the treated lysates were further divided into smaller aliquots (50 l), which were then heated at different temperatures for 3 min on a Veriti thermal cycler (Applied Biosystems) followed by cooling for 3 min to room temperature

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Here, our systematic survey study with several membrane proteins demonstrates that TGP is indeed a robust and reliable FSEC-TS reporter as it enables assays at ~90 C with T m values in close agreement with established methods

N-acetylcysteine exposure is associated with improved survival in anti-nuclear antibody seropositive patients with usual interstitial pneumonia