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Th2/Tc2 cells can be differentiated under IL-4 conditions and produce IL-4, IL-5 and IL-13 through GATA-3 and GATA-3, STAT6, respectively ( Th17 cells are characterized by the release of proinflammatory cytokines, such as IL-17A, IL-17F, IL-21 and IL-22, which are associated with COPD progression and the exacerbation of alveolar destruction ( Studies over the past decade has emphasized the importance of maintaining a balance between Th17 cells and Treg cells in controlling the inflammatory response in COPD ( T-lymphocyte-mediated dysfunction of cellular immunity may have an impact in the course of AECOPD, participating in and contributing to its deterioration ( 3.5.2 B lymphocytes In the lungs of COPD patients, the number of B-lymphocytes is increased, especially in patients with severe disease ( 3.6 Dendritic cells Dendritic Cells (DCs) also play an important role in the pathogenesis of COPD
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From the master plates, 5 ng of DNA was used for a conventional PCR to amplify HV2 region
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