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Cholangiocyte organoid culture For cholangiocyte organoid cultures, EpCAM-positive cholangiocytes were mixed with Matrigel growth factor reduced (Matrigel, Corning) or Cultrex basement membrane extract 2 (BME2) (Cultrex-RGF basement membrane extract type 2, BME2 (AMSBIO) at 50,000 cells per 50 l in each well of a 24-well plate and cultured at 37 C and 5% CO 2 in h-CholOrg EM medium as described in refs

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6.3 Toward personalized and mechanism-driven functional eggs Future progress in functional egg development will likely require a transition from population-level fortification to mechanism-driven and personalized nutrition strategies tailored to specific life stages and health conditions, such as aging, pregnancy, and metabolic disorders