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Always aim at the glass wall and let water roll down to the powder

Stimulation assays for testing the effects of activity on the localization of SVs Surface epitopes of Syt1 were saturated by incubating the neurons in divalent-free buffer (Tyrode buffer without Mg 2+ and Ca 2+ ) with HaloTag-free NbLumSyt1 for 5 min at 37 C