After overnight incubation, the medium was changed to 90% of Williams E Medium and supplemented with 12.5% HepaRG preinduction and toxicity medium supplement (Lonza, catalog: MHPIT) and 25 U/mL penicillin-25 g/mL streptomycin, followed by 72 h incubation
[DOI] [PubMed] [Google Scholar] 36.Walsh C.J., Zaihra T., Benedetti A., Fugre C., Olivenstein R., Lemire C., Hamid Q., Martin J.G
Yes, blocking total androgen production significantly decreases androgen receptor activity (likely because there are fewer total androgens available)
GSH synthesis using the whole-cell biocatalyst was performed in 0.2 M phosphate buffer (pH 7.0) containing 40 mM l-glutamate, 20 mM l-cysteine, 40 mM glycine, 20 mM MgCl 2 and 20 mM ATP