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In the 1960s consumers became more aware that smoking leads to cancer, and in 1965 legislation passed that mandated that cigarette packs and advertisements have warnings

Studies in mice investigating the effects of e-cigarettes on different brain regions have found that e-cigarette exposure during pregnancy can alter the transcriptome of the frontal cortex (1316 mg/mL nicotine), increase global DNA methylation within the brain (1316 mg/mL nicotine), and dysregulate gene expression in the hippocampus (13 mg/mL nicotine) [176]