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Poor membrane health is associated with chronic illness

Cell pellets were resuspended in reducing lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 10 mM DTT, 0.01 mg ml 1 DNase I) to an OD 600 of 140 and lysed by two rounds of sonication for 2 min at 50% amplitude with 2 s intervals using a Sonopuls (Bandelin) sonicator

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Current support for ferroptosis as a primary driver of AD pathogenesis remains largely correlative, relying on post-mortem analyses, animal models, and in vitro data