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Also, the Fmocbased method may be considered resourceintensive and environmentally harmful

Chromatographical separation of metabolites was performed on Syncronis HILIC UHPLC column (2.1 mm 100 mm 1.7 m, Thermo Fisher) at the flow rate of 600 L/min using a binary solvent system: solvent A, 20 mM ammonium formate, pH 3.0 in mass spectrometry grade H 2 0, and solvent B, mass spectrometry grade acetonitrile with 0.1% formic acid (%v/v)

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After 68 weeks, weight loss was higher with one-weekly subcutaneous semaglutide 2.4 mg compared with once-daily liraglutide 3.0 mg ( 15.8% vs