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Instead of continuing to analyse smoking and HPV as oncogenic factors separately, we set out to focus on their possible interaction, said Enrique Boccardo, penultimate author of the article and a professor in the Department of Microbiology at the University of So Paulos Biomedical Sciences Institute (ICB-USP) in Brazil.Both smoking and HPV are associated with heightened oxidative stress and DNA damage, which are factors in cancer and, according to previous research, can regulate superoxide dismutase 2 [SOD2], a putative biomarker of oral cancer malignancy and other diseases associated with HPV. The researchers first conducted in vitro experiments to analyse oral cells that expressed the oncoproteins HPV16 E6 and E7 [showing infection by HPV] and were exposed to cigarette smoke condensate

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Males had a higher prevalence of both ever and current e-cigarette use than females