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The lowest concentration which elicited a significantly different response compared to the control was identified as the Lowest Observed Effect Concentration (LOEC), whilst the highest concentration which did not cause a significantly different response compared to the control was identified as the No Observed Effect Concentration (NOEC)

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He escalated the protest in 1766 by writing the Westmoreland Resolves, which promised opposition to the Stamp Act at every hazard, and, paying no regard to danger or to death. Further, anyone who attempted to enforce it would face immediate danger and disgrace. The signatories, prominent citizens of Westmoreland County, Lees home, pledged that they would refuse to purchase British goods until the Stamp Act was repealed