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ELISA assays complement the HPLC analysis by measuring concentrations of melatonin and neurotrophic factors, utilizing kits from Sangon Biotechnology Company (Shanghai, China) following strict protocols to ensure reliability

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All cell lines were regularly tested for mycoplasma contamination using MycoAlert Mycoplasma Detection Kit (Lonza) and by assessment of (lack of) DAPI-positive microvesicles under fluorescence microscope