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Oral MTX responsiveness can be enhanced by administering a large starting dose and rapidly titrating the medication

Small molecule absorption becomes a particularly important issue for working with BBB models, as the BBB is a highly selective cell barrier, which often only allows small lipophilic molecules with a molecular weight below 400 Da to pass through a paracellular route 49,50,51 Therefore, the most interesting compounds for treating central nervous system (CNS) diseases often lie in the range of molecules readily absorbed by PDMS

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