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Glutamate may also induce intra-articular inflammatory responses and abnormal pain in OA patients by activating N-methyl-D-aspartic acid (NMDA) receptors, further advancing OA progression (Piepoli et al., 2009)

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Briefly, HEK293T cells, cultured to approximately 90% confluence on the day of transfection, were subjected to transfection using Lipofectamine 3000