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The cell cycle was studied by flow cytometry, using the level of the fluorescence of the DNA dye propidium iodide (final concentration, 5 g/mL) at 630 nm fluorescence emission as a measure of the DNA content per cell, as described in materials and methods

Nano encapsulation has been applied to enhance bioavailability, preserve the integrity of bioactive ingredients, increase half-life of the bioactive ingredients, reduce toxicity and side effects, target delivery to specific sites as well as improve the absorption and efficacy of bioactive ingredients

However, preterm infants still have a high risk of developing complications and poor outcomes, and this risk is even higher for infants born below their gestational age