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For instance, treatment of subarachnoid hemorrhage with bone marrow mesenchymal stem cells resulted in upregulation of CHAC1/BOTCH , which significantly reduced neuroinflammation by blocking NOTCH1 signaling (Li et al., 2019

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Membranes were stripped for 20 min at room temperature using a Blot Restore Membrane Rejuvenation kit (Sigma-Aldrich, 2520-M), then washed twice with TBS-T, re-blocked with BSA 5% for 30 min and incubated with mouse-anti--actin (Sigma-Aldrich, A1978) 1:3,000 in BSA 2.5% for 4 h at room temperature and then incubated with a sheep-anti-mouse (Amersham, NA931) secondary antibody at 1:5,000 in BSA 2.5% for 2 h

Raw garlic: Crushed or macerated and left out to sit for about 10 mins is the best way to use garlic in food